Salinivibrio sp.YH4胞外丝氨酸蛋白酶EYHS耐盐性及生物信息学分析          下载免费PDF全文

武翠玲  宋英达  高慧芳  张廉芸  任晨霞 《盐湖研究》2020,28(1)

摘要：目的 探讨菌株Salinivibrio sp.YH4分泌的丝氨酸蛋白酶EYHS的耐盐性及结构特征。方法 明胶底物酶谱法分析EYHS的耐盐性。应用生物信息学手段对EYHS及6种耐盐的S8家族丝氨酸蛋白酶结构特征进行分析。结果 EYHS在4 mol/L的NaCl溶液中仍具有活性，属于耐盐蛋白酶。EYHS及6种S8家族丝氨酸蛋白酶分子表面的loop区等无规则卷曲所占比例较高，α-螺旋与β-片层则主要位于酶分子内部。EYHS分子表面酸性氨基酸含量较高，且具有弱疏水内核。多序列比对发现蛋白酶的催化三联体两侧存在高度保守的基序和保守的极性氨基酸及芳香族氨基酸，并存在多个保守的Gly与Ala。同源模建和表面电荷分布显示，α螺旋和β片层围成了蛋白酶的催化腔，EYHS活性中心包含由Asp32、His65与Ser215组成的催化三联体，且催化位点区域表面静电势为负。结论 上述结构特征可能有助于耐盐丝氨酸蛋白酶EYHS在高盐环境下维持其稳定性和适度柔性，并有助于其催化功能的发挥，为深入研究耐盐丝氨酸蛋白酶的高盐环境适应性提供了一定的理论依据。  相似文献   

Correspondence between the large volcanic eruptions and ENSO events over AD 1525–2000     

Fang  Xiuqi  Zheng  Xue  Zhang  Xing 《地理学报(英文版)》2020,30(1):103-118

ENSO is an interannual mode which may be affected by external forcing, such as volcanic eruptions. Based on the reconstructed volcanic eruptions chronology and ENSO sequences, both 195 large volcanic eruptions(VEI≥4) and 398 ENSO(El Ni?o and La Ni?a) events were extracted from 1525 to 2000. An analysis of the correspondence between the large volcanic eruptions and ENSO events was performed by matching the large volcanic eruptions with the types and magnitudes of ENSO events present in the 0–2 years after the eruptions. The results show the following:(1) The percentages of ENSO events within the 3 years after the large eruptions had increased to 68.3% from 31.7% compared with those with no-eruptions in the previous 0–2 years. In addition, the ratio of El Ni?o to La Ni?a events turned from 2:3 to 1:1, and more El Ni?o events occurred in the 0 year after eruptions in the low-latitudes of the Northern Hemisphere and in the tropics but more La Ni?a events occurred in the 0 year after in the high-latitudes of the Northern Hemisphere and the Southern Hemisphere.(2) After the eruptions, the weak(W) El Ni?o events had increased by 8 percentage points and the very strong(VS) El Ni?o events had decreased by 10 percentage points; conversely, there was a decrease by 15 percentage points of the weak La Ni?a events and an increase by 11.4 percentage points of the very strong La Ni?a events. Specifically, the percentages of strong La Ni?a events increased to a peak at 1(+1) year after the eruptions.(3) The percentage of eruptions followed by single-year ENSO was the greatest. The percentage of ENSO events that occurred in the consecutive 2 years following an eruption was approximately equal to the percentage of events that occurred consecutively 3 years following an eruption, and both sets of ENSO magnitudes showed a decreasing trend.  相似文献   

敞开酸溶和偏硼酸锂碱熔ICP-MS法测定多金属矿中的稀土元素及铌钽锆铪   总被引：2，自引：2，他引：0  

门倩妮  沈平  甘黎明  冯博鑫 《岩矿测试》2020,39(1):59-67

用电感耦合等离子体质谱法(ICP-MS)测定地质样品中的稀土及难熔元素,混合酸敞开酸溶法和碱熔融法是两种主要的溶样方法。但地质样品组分复杂,元素之间存在相互共生的现象,对于特殊元素、特殊样品用传统酸溶法会造成部分元素消解不完全,使测定结果不准确;而碱熔法的操作过程繁琐,且溶液盐度高,易产生基体干扰和堵塞仪器进样系统。本文改进了传统四酸和五酸体系,采用氢氟酸-硝酸-硫酸敞开酸溶体系,用国家一级标准物质制作标准曲线测定15种稀土元素,方法准确度(ΔlgC)为0.001～0.027。同时改进了偏硼酸锂碱熔法,样品用偏硼酸锂碱熔提取,加入氢氧化钠调节溶液至碱性条件,所测元素与偏硼酸锂共沉淀后过滤分离熔剂,再用硝酸复溶测定15种稀土元素及铌钽锆铪。两种溶样方法的测定值与认定值的相对误差为1.09%～9.30%。将混合酸敞开酸溶法测定稀土元素、偏硼酸锂碱熔法测定铌钽锆铪的结果与其他实验室密闭酸溶法相比,两组数据的相对偏差为0.13%～15.32%。本实验表明,混合酸敞开酸溶法适用于测定地质样品中的稀土元素,偏硼酸锂碱熔法不仅适用于测定地质样品中的稀土元素及铌钽锆铪,也适用于测定如古老高压变质岩石及铝含量高的样品中的铌钽锆铪。  相似文献   

Conjugate ruptures and seismotectonic implications of the 2019 Mindanao earthquake sequence inferred from Sentinel-1 InSAR data     

《International Journal of Applied Earth Observation and Geoinformation》2020

In 2019, four strong earthquakes of Mw>6.4 occurred successively in Mindanao, Philippines. Based on the reports from the USGS and PHIVOLCS, these earthquakes were dominated by strike-slip ruptures. Whether these earthquakes are temporally and spatially related remained unknown. We characterized the coseismic displacement fields during the earthquake sequence using an InSAR technique with Sentinel-1 SAR data. The InSAR deformation measurements convincingly reveal that the four earthquakes produced distinct coseismic displacement patterns. We estimated the source parameters of the earthquakes with a two-step inversion strategy. The optimal model suggests that the earthquake sequence resulted from the reactivation of a conjugate fault structure that involves two nearly vertical left-lateral strike-slip faults and two high-angle right-lateral strike-slip faults. We calculated Coulomb stress changes from the earthquake sequence, suggesting that the previous strong earthquakes had significant stress-encouraging effects on the following events. The regional velocities based on the GPS analysis suggest that the formation of this conjugate structure is mainly due to the westward movement of the subducting Philippine Sea Plate. This earthquake sequence provides a seismotectonic background for subsequent strong earthquakes and helps to better understand the formation mechanisms and seismotectonic implications of conjugate structure rupturing.  相似文献   

九寨沟Ms7.0地震的同震形变场误差研究及断层滑动分布反演     

庄春晓  王仕祥  王仲想 《测绘与空间地理信息》2020,(3):211-214,224

北京时间2017年8月8日四川省九寨沟发生Ms7.0级地震。为了提高断层滑动分布反演的可靠性,本文利用升降轨InSAR数据联合反演断层滑动分布,再根据模拟形变值二次反演,对比反演结果来探讨升降轨形变场误差和确定断层滑动分布的结果。结果表明,九寨沟升轨同震形变场的质量较好,降轨形变场受余震、震后粘弹性松弛效应影响明显,利用模拟形变值二次反演确定的断层滑动分布可靠性更高。  相似文献   

浑河断裂地球物理场特征及地震活动性     

张鐘月  万波  彭虎  田德欣 《世界地质》2020,39(1):176-184

浑河断裂是辽宁省内一条重要的北东向地震构造带,断裂两侧的地球物理重磁场存在差异。通过浑河断裂的区域重力场与磁场研究,认为浑河断裂既控制了深、浅部岩石地层的发育,同时浑河断裂的重力场、磁场分布随着深、浅部地层岩性发育的差异而有所变化。浑河断裂的重力场和磁场梯度带主要位于抚顺以西至沈阳一带,浑河断裂历史以来有记录的最大破坏性地震位于重力梯度变化较大的沈阳段。综合浑河断裂地球物理场特征及区域地质资料,浑河断裂虽然活动性一般,但抚顺地区的采煤活动对断裂的活动性有一定影响。近些年抚顺地区频发的沉降、滑坡和地裂缝等地质灾害应当引起对浑河断裂活动性研究的重视。  相似文献   

The δ60/58Ni Values of Twenty‐Six Selected Geological Reference Materials     

Weihan Li  Jian‐Ming Zhu  Decan Tan  Guilin Han  Zhouqiao Zhao  Guangliang Wu 《Geostandards and Geoanalytical Research》2020,44(3):523-535

The high‐precision δ^60/58Ni values of twenty‐six geological reference materials, including igneous rocks, sedimentary rocks, stream sediments, soils and plants are reported. The δ^60/58Ni values of all samples were determined by double‐spike MC‐ICP‐MS (Nu Plasma III). Isotope standard solution (NIST SRM 986) and geological reference materials (BHVO‐2, BCR‐2, JP‐1, PCC‐1, etc.) were used to evaluate the measurement bias and intermediate precision over a period of six months. Our results show that the intermediate precision of Ni isotope determination was 0.05‰ (2s, n = 69) for spiked NIST SRM 986 and typically 0.06‰ for actual samples, and the δ^60/58Ni _{NIST SRM 986} values were in excellent agreement with previous studies. Eighteen high‐precision Ni isotope ratios of geological reference materials are first reported here, and their δ^60/58Ni values varied from ?0.27‰ to 0.52‰, with a mean of 0.13 ± 0.34‰ (2s, n = 18). Additionally, SGR‐1b (0.56 ± 0.04‰, 2s), GSS‐1 (?0.27 ± 0.06‰, 2s), GSS‐7 (?0.11 ± 0.01‰, 2s), GSD‐10 (0.46 ± 0.06‰, 2s) and GSB‐12 (0.52 ± 0.06‰, 2s) could potentially serve as candidate reference materials for Ni isotope fractionation and comparison of Ni isotopic compositions among different laboratories.  相似文献   

地震后水汽异常变化初探     

王勇  任栋  娄泽生  郝振航 《大地测量与地球动力学》2019,39(9):881-883

研究东日本地震、汶川地震和玉树地震震中及其附近区域在地震前后的水汽时间序列变化。首先分析震中MODIS水汽序列和震中附近探空站点水汽序列在地震前后的变化；然后基于GNSS ZTD与水汽之间的高相关性，以GNSS ZTD代替GNSS水汽，讨论震源区周围IGS站点的ZTD序列变化。研究发现，震后震中及其附近区域水汽值变化出现异常，且距离越近所受影响越大；水汽不断聚积，达到峰值后发生降水。  相似文献   

茅山竹矿断层气定点观测场地勘选     

戴波  吴晓峰  张敏  孟科 《大地测量与地球动力学》2019,39(11):1202-1206

拟在茅山断裂带沿线布设若干个断层气连续观测点，建组断层气观测网。以茅山竹矿地区为例，通过断层气测量的初步结果，结合地质、人工地震、跨断层短水准等多种手段联合分析研究区断层的活动性，从而确定断层气定点观测的点位，为组建断层气观测网的选点提供借鉴和参考。  相似文献   

利用InSAR资料反演宁洱Ms6.4地震震源参数及库仑应力变化     

陈晨月  冯光财  高华  杨怀宁 《大地测量与地球动力学》2019,39(3):295-301

首先利用ALOS PALSAR数据，通过D-InSAR技术获取2007-06-03云南宁洱MS6.4地震的同震形变场，然后基于Okada弹性半空间位错模型反演该地震的断层几何以及精细滑动分布，最后计算宁洱地震后周边断层的静态库仑应力变化。结果表明，形变主要集中在西盘，最大视线向形变量为51.6 cm；反演得到的震源位置为23.05°N、101.02°E，深度3 km，断层走向145°，倾向49.5°，平均滑动角153°，发震断层为NNW向普洱断裂，断层活动以右旋走滑为主，兼具逆冲分量；断层面最大滑动量为1.2 m，反演得到的震级为MW619。基于库仑应力场发现，磨黑断裂处于库仑应力增加区域，而2014年景谷地震位于负值区域。结合实地考察资料和反演结果表明，宁洱地震为浅源地震，但断层并未出露地表。  相似文献